VDSpher® MS Phases

Mass spectrometry is becoming more and more crucial as an online detection method. The requirements of the LC/MS analytics are reflected in the conception of HPLC columns. To reduce the solvent quantity and so as not to endanger the vacuum created in the mass spectrometer, columns of smaller dimensions are normally preferred. To profit from a high resolution further, silica gel with a small particle size is recommended for the column. VDSpher MS satisfies this requirement with 2.5 µm separation phases. If the resolution is not crucial, VDSpher MS columns with 4 µm silica gels are also available.

In principle, any VDSpher column can be used for LC/MS. Because as a sensitive detection method, mass spectrometry is in a position to display very low impurities, depending on the application e.g., carry-overs from previous runs (“carry-over effect“). VDSpher MS phases are selected specially to avoid these effects. In all, ten different modifications are available. The C18 phases enable a large bandwidth of hydrophilic (C18-H, C18-LC-H) and hydrophobic (C18-DE, C18-B-DE) applications. The not endcapped phase C18-B has medium hydrophobicity. Additionally, there are hydrophobic VDSpher MS phases with C8, C4, Phenyl and CN modifications.

VDSpher MS phases are available with a pore size of 100 Å. The VDSpher MS 100 normal and reversed phase modifications are shown in the Table.

Normal and Reversed Phase Modifications: VDSpher® MS 100

Phase Endcapping Carbon content [%] pH range USP Code
VDSpher® MS 100 C18-DE yes 17.2 2 - 9 L1
VDSpher® MS 100 C18-B no 17.7 2 - 7.5 L1
VDSpher® MS 100 C18-B-DE yes 20.1 2 - 11 L1
VDSpher® MS 100 C18-H polar 11.5 2 - 7.5 L1
VDSpher® MS 100 C18-LC-H hydrophilic 13.5 2 - 8 L1
VDSpher® MS 100 C8-B-DE yes 11.2 2 - 10 L7
VDSpher® MS 100 C4-B-DE yes 7.3 2 - 9 L26
VDSpher® MS 100 Phenyl-DE yes 10.9 2 - 9 L11
VDSpher® MS 100 CN-DE yes 7.2 2 - 9 L10
VDSpher® MS 100 CN-DE-RP yes


2 - 9 L10
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